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mouse cdna library  (TaKaRa)


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    Structured Review

    TaKaRa mouse cdna library
    Mouse Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+cdna/Mouse+Kidney+Matchmaker+cDNA+Library+pACT2/pmc11668847-53-10-13
    Average 93 stars, based on 49 article reviews
    mouse cdna library - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Human cytomegalovirus UL141 protein interacts with CELF5 and affects viral DNA replication.
    Article Snippet: Yeast two-hybrid experiments were performed according to the manufacturer's protocol using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech Laboratories, Inc.). .. Plasmid pGBKT7-UL141 containing the GAL4 DNA-binding domain was transformed into Saccharomyces cerevisiae AH109 (Clontech Laboratories, Inc.,) containing a human fetal brain cDNA library, pACT2-cDNA. .. The human fetal brain cDNA library was provided by Professor Gengfu Xiao from the State Key Laboratory of Virology, College of Life Sciences, Wuhan University (Wuhan, China), which was cloned into pACT2 (Clontech Laboratories, Inc.) by electroporation (Bio-Rad Laboratories, Inc.) according to the manufacturer's protocol in BL21 (DE3) cells (Tiangen Biotech Co., Ltd., Beijing, China) at a density of 6x107 cells/ml in logarithmic growth period.

    Transformation Assay:

    Article Title: Human cytomegalovirus UL141 protein interacts with CELF5 and affects viral DNA replication.
    Article Snippet: Yeast two-hybrid experiments were performed according to the manufacturer's protocol using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech Laboratories, Inc.). .. Plasmid pGBKT7-UL141 containing the GAL4 DNA-binding domain was transformed into Saccharomyces cerevisiae AH109 (Clontech Laboratories, Inc.,) containing a human fetal brain cDNA library, pACT2-cDNA. .. The human fetal brain cDNA library was provided by Professor Gengfu Xiao from the State Key Laboratory of Virology, College of Life Sciences, Wuhan University (Wuhan, China), which was cloned into pACT2 (Clontech Laboratories, Inc.) by electroporation (Bio-Rad Laboratories, Inc.) according to the manufacturer's protocol in BL21 (DE3) cells (Tiangen Biotech Co., Ltd., Beijing, China) at a density of 6x107 cells/ml in logarithmic growth period.

    cDNA Library Assay:

    Article Title: Human cytomegalovirus UL141 protein interacts with CELF5 and affects viral DNA replication.
    Article Snippet: Yeast two-hybrid experiments were performed according to the manufacturer's protocol using the Matchmaker GAL4 Two-Hybrid System 3 (Clontech Laboratories, Inc.). .. Plasmid pGBKT7-UL141 containing the GAL4 DNA-binding domain was transformed into Saccharomyces cerevisiae AH109 (Clontech Laboratories, Inc.,) containing a human fetal brain cDNA library, pACT2-cDNA. .. The human fetal brain cDNA library was provided by Professor Gengfu Xiao from the State Key Laboratory of Virology, College of Life Sciences, Wuhan University (Wuhan, China), which was cloned into pACT2 (Clontech Laboratories, Inc.) by electroporation (Bio-Rad Laboratories, Inc.) according to the manufacturer's protocol in BL21 (DE3) cells (Tiangen Biotech Co., Ltd., Beijing, China) at a density of 6x107 cells/ml in logarithmic growth period.



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    Image Search Results


    Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: Y2H cDNA library construction and screening procedure. A) The conventional procedure for cDNA library generation. B) Using the cDNA library in Y2H screens to identify protein–protein interactions. C) Using the cDNA library to screen for rescue genes involved in DNA repair. D) Screening randomly selected plasmids from the cDNA library to screen random samples from the library “Created with Biorender.com .”.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: cDNA Library Assay, Protein-Protein interactions

    The 5′ end truncated cDNAs identified, bp; base pairs.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: The 5′ end truncated cDNAs identified, bp; base pairs.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: Variant Assay, Binding Assay, RNA Binding Assay

    mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: mRNA priming in cDNA libraries. A) Normal priming through reverse transcriptase elongation of cDNA after oligo (dT) binding to true poly-A tails. B) Internal priming that leads to the generation of truncated transcripts due to internal A-rich regions and reverse transcriptase stalling. “Created with Biorender.com .”.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: Reverse Transcription, Binding Assay

    Mitochondrial hits status and their quantification.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Insights into the human cDNA: A descriptive study using library screening in yeast

    doi: 10.1016/j.jgeb.2024.100427

    Figure Lengend Snippet: Mitochondrial hits status and their quantification.

    Article Snippet: The Human Kidney MatchmakerTM cDNA Library was purchased from Clontech (Cat. No 638816).

    Techniques: